NPDN Diagnostic Tips
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P&V Quick tips
Triage
- Turfgrass triage 8/2020
 - Mushroom Identification 01/2021
 - Common Needle Diseases and Disorders of Spruce and Pine 4/2021
 
Molecular techniques
- Tissue Homogenization for Nucleic Acid Extraction Tips 2/2021
 - Synthetic Positive Control 4/2020
 - Working with synthetic oligos 5/2020
 - Coping with PCR inhibitors 12/2020
 
Serology
- Tips for ELISA success. 6/2020
 
Organism specific. Morphology/Microscopy/Barcoding
- Tips for Identifying Fusarium. Part 1: Morphological Identification 5/2021
 - Tips for identifying Rhizoctonia 3/2021
 
NPDN Newsletter Diagnostic Tips - 2018 and older
- Table grapes as baits for Botrytis, 1/18
 - The Use of a French Cooking Technique, Chiffonade, To Improve Accuracy for Virus Testing, 2/14
 - New E-Gro diagnostic resources released, 1/14
 - Quick tip: a hammer and solid surface can be used for fast, easy pulverization 10/13
 - Suggested Online Resources for Determining “First Report Status” 9/13
 - From the Archives: Using Cheap Filters to Soften Lighting Hot Spots 8/13
 - Free TurfPath app 6/13
 - Another Easy Transfer Tool for Fungi 5/13
 - Useful Literature References 3/13
 - Transfer Tools for Fungi 2/13
 - Solution for Clamping Branches in the Diagnostic Laboratory 1/13
 - Sample Photography as a Standard Operating Procedure 12/12
 - Using a Hammer and Chisel to Isolate From Large Branches and Trunks 11/12
 - 3-D Riker Mounts for Bulky Samples 10/12
 - Tips for Isolating Phialophora gregata, the Brown Stem Rot (BSR) Pathogen, 8/12
 - How we test for rose rosette in Oklahoma 7/12
 - National Dothistroma Collection – Sampling & Shipping Protocol 6/12
 - What Do Pachysandra and Boxwood Have in Common? 3/12
 - Deep Well Projection Slides — A Useful Tool for Microscope Work - 12/11
 - Use of Autoclaved Pine Needles for Inducing Sporulation of Botryosphaeria Isolated from Plant Tissue - 11/11
 - Septoria lycopersici Isolation from Tomato Leaves - 10/11
 - Tip for Using Liquid Nitrogen and a Mini-BeadBeater-1 for Tissue Disruption - 9/11
 - Extending the Working Life of Diagnostic Samples - 7/11
 - Timely Tips for Triage Time - 6/11
 - Isolation Methods for Obtaining Endophytic Tubakia spp. from Oak Twigs - 4/11
 - Advantages of Using FTA Cards in the Lab - 3/11
 - Diagnostics and Educational Outreach - 2/11
 - Inducing Phomopsis to Produce Beta Spores - 1/11
 - Peppers as an Alternative to Potato Slices for Bacterial Soft Rot Bioassay - 12/10
 - Loop-mediated Isothermal Amplification (LAMP) as a Diagnostic Tool - 11/10
 - Use of Plastic Coverslips for ‘Squash’ Mounts - 10/10
 - Use of a Multi-functional Copier as a Diagnostic Tool - 9/10
 - Method for Isolating and Maintaining Cultures of Geosmithia from Juglans nigra - 8/10
 - Tobacco Hypersensitivity - 7/10
 - Diagnosis of Powdery Scab (Spongospora subterranea f.sp. subterranea)- 6/10
 - Immunosorbent Electron Microscopy (ISEM) Using Crude Plant Extract for Detection of Plant Viruses- 5/10
 - A Technique for Diagnosing Aphanomyces Root Rot on Alfalfa Seedlings- 4/10
 - Detecting Systemic Infections of Downy Mildew in Broccoli 3/10
 - Submitting Greenhouse Samples- 2/10
 - A Novel Approach to Testing with Immunostrips- 11/09
 - A Simple Diagnostic Technique for Identification of Three Wheat Rust Fungi- 09/09
 - Clearing and Staining Roots and Other Plant Tissues- 7/09
 - Quick Sectioning Technique- 6/09
 - Preparation and Storage of Primers and Probes- 5/09
 - Long-term Freezer Storage of Didymella bryoniae on Filter Paper- 4/09
 - Forceps Tip- 3/09
 - Using Window Screening as a Strainer for Isolation Material- 2/09
 - Two Techniques Enhancing Detection of Sporulating Fungi- 1/09
 - Using Cheap Filters to Soften Lighting- 12/08
 - Detecting Phytophthora in Soil- 11/08
 - Repointing Forceps- 10/08
 - Improving Isolation Results- 9/08
 - Tools to Ease SCN Egg Extractions- 7/08
 - Obtaining Pure Bacterial Colonies- 6/08
 - Double-Stranded RNA- 5/08
 - Parafilm Dispenser- 4/08
 - Moist Chamber on Water Agar- 3/08
 - Soybean Dwarf Testing- 2/08
 - Testing Races Aphanomyces- 1/08
 - Carrot Bait for Thielaviopsis- 12/07
 - Clinic Floor Plans- 11/07
 - Pure Culture Fungal Leaf Spots- 10/07
 - Inverted Microscope- 9/07
 - Parafilm- 8/07
 - Incubating Tips- 7/07
 - Oak Wilt Testing- 6/07
 - Bacterial Streaming- 5/07
 - Forceps Technique- 4/07
 - Float Incubation- 3/07
 - Clear Tape Technique- 2/07
 
      