Event

• Dates offered: March 27 – 28, 2023
• Maximum course enrollment: 8
• Length of time: 2 days - in-person

The citrus canker molecular detection workshop is being offered in person for the first time in 2023. The workshop will have lecture and hands-on components. The lectures will cover the disease characteristics and an overview of citrus canker diagnostic testing at the USDA-APHIS-PPQ S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL).

• Dates offered: Two Sessions: March 16-17, 2023 or March 23-24, 2023
• Maximum course enrollment: 8 per session
• Length of time: 2 days per session - in-person

The isothermal amplification workshop for 2023 will be offered in-person and will have lecture and hands-on components.

• Dates offered: Two Session: March 6-8, 2023 or March 20-22, 2023
• Maximum course enrollment: 8 per session
• Length of time: 3 days per session - in-person

The Phytophthora 101 Workshop is a 3-day workshop that has lectures and hands-on components. The lectures include a review of Phytophthora ramorum, DNA extraction, the real-time PCR protocols using P. ramorum (ITS and Elicitin) and Phytophthora kernoviae (ITS1 and ITS2) as the targets, and interpretation of results.

• Dates offered: Two Sessions: March 16-17, 2023 or March 23-24, 2023
• Maximum course enrollment: 8 per session
• Length of time: 2 days per session - in-person

The isothermal amplification workshop for 2023 will be offered in-person and will have lecture and hands-on components.

• Dates offered: March 14-15, 2023
• Maximum course enrollment: 8
• Length of time: 2 days - in-person

The Phytoplasmas Molecular Detection Workshop is a 2-day workshop that has lectures and hands-on components. The lectures include an introduction to phytoplasmas, their taxonomy and the disease symptoms associated with some of these bacterial pathogens of interest to APHIS PPQ.

• Dates offered: March 9-10, 2023
• Maximum course enrollment: 8-10
• Length of time: 2 days - in-person

Day 1: Lecture & Demonstrations & Lab Exercise
Day 2: Laboratory Exercise

• Dates offered: Two Session: March 6-8, 2023 or March 20-22, 2023
• Maximum course enrollment: 8 per session
• Length of time: 3 days per session - in-person

The Phytophthora 101 Workshop is a 3-day workshop that has lectures and hands-on components. The lectures include a review of Phytophthora ramorum, DNA extraction, the real-time PCR protocols using P. ramorum (ITS and Elicitin) and Phytophthora kernoviae (ITS1 and ITS2) as the targets, and interpretation of results.

• Dates offered: March 2, 2023
• Maximum course enrollment: 20
• Length of time: 1 day - virtual

The TaqMan probe real-time PCR basics workshop is being offered for the first time in 2023.

• Dates offered: February 27, 2023 – March 01, 2023
• Maximum course enrollment: 20
• Length of time: 3 days - virtual

The Bioinformatics Module SS workshop is taught over 3 days and has both lecture and hands-on activities. The lectures will cover a range of sanger sequencing topics from primer design to introductory sequence analysis, as well as the molecular classification and diagnostics of a wide range of pathogens including bacteria, fungi, nematodes, Phytoplasmas, viruses and Phytophthora species.

• Dates offered: February 21, 2023
• Maximum course enrollment: unlimited
• Length of time: 2-3 hours - virtual

The good laboratory practices and quality management systems workshop is being offered for the first time in 2023.  The workshop will be held virtually and will be a prerequisite for all other NPDN trainings this year.  The quality management portion of the lecture will discuss quality management systems vs ISO 17025 accreditation and the importance of record management in all aspects of quality management.  The g