About the Workshop

This 3-day hands-on workshop will focus on molecular methods used in plant diagnostic laboratories, using bacterial plant pathogens. The goal of this training is to strengthen diagnostic capacity by combining practical laboratory exercises with expert-led instruction on molecular methods and bacterial diagnostics.

Location

UF Plant Diagnostic Center
University of Florida
Gainesville, FL

Dates

February 24–26, 2026.

Participants can expect to learn about:

  • Molecular detection and identification of bacterial plant pathogens
  • Sample preparation considerations for bacterial diagnostics
  • PCR- and sequencing-based methods used in diagnostic workflows
  • Interpretation of molecular results in a diagnostic context
  • Troubleshooting common challenges in molecular diagnostics
  • Integration of molecular tools with traditional diagnostic methods
  • The workshop will be taught by multiple subject matter experts from across the diagnostic network. 

Subject Matter Experts 

Molecular- Tom Rounsville and Olivia Choi, University of Maine 

Bacterial- John Bonkowski, Purdue University

Agenda

Updated 1/23/2026

Three-day workshop schedule shown in a table with times on the left and sessions for Tuesday, February 24; Wednesday, February 25; and Thursday, February 26. Tuesday covers plant pathogenic bacteria and qPCR fundamentals, Wednesday focuses on qPCR plate prep, data analysis, and troubleshooting, and Thursday covers DNA sequencing, BLAST, alignments, and wrap-up sessions, with lunch breaks and optional evening discussions each day.